We also assessed the expression of apoptotic regulators, Bax and Bcl-2, and found that overexpression of ZHX1 in GBM (T98G and U251) cells was associated with decreased expression of Bax (pro-apoptotic protein) and increased expression of Bcl-2 (anti-apoptotic protein), whereas knockdown of ZHX1 produced the opposite effect (Figure 2; p < 0.01). This evidence concerns the gene ZHX1 and glioblastoma.