To explore the effect of PPP2R3A gene knockdown on the malignant behaviors of liver cancer cells, we constructed two shRNA lentiviral vectors, namely shRNA‐PPP2R3A‐6328 (shRNA1) and shRNA‐PPP2R3A‐6332 (shRNA2), to infect two liver cancer cell lines, Huh‐7 and HepG2, individually. This evidence concerns the gene PPP2R3A and liver cancer.