There were no differences between HF and LF in the concentrations of insulin (trial × time, p = 0.503; trial, p = 0.284; time, p < 0.001; Fig. 2c), but the plasma insulin AUC was higher in the HF trial than in the LF trial (p = 0.015; Fig. 2d). This evidence concerns the gene INS and hydrops fetalis.