These findings also suggest that commercial ELISA kits for anti-ZIKV IgM can be a good diagnostic test for ZIKV infection in Taiwan and probably in other dengue non-endemic countries since cross-reactivity is of less concern; however, serological tests for ZIKV-specific IgG still remain a challenge, even in dengue non-endemic countries. This evidence concerns the gene CD40LG and Zika virus infectious disease.