In view of the overexpression of EMT related protein vimentin as well as Chr8 aneuploidy in tumor cells predict poor prognosis in many carcinomas including lung cancers (Du et al. 2018; Karim et al. 2017), both karyotypic and phenotypic of CTCs from patients with newly diagnosed advanced lung cancer were evaluated by SE-iFISH platform in current prospective study. The gene discussed is VIM; the disease is lung cancer.