Mutation or absence of TSC1 led to hypoacetylation of Hsp90 and this was demonstrated in Hsp90 isolated from four cell types lacking TSC1: 1) bladder cancer cells with TSC1 mutation, 2) TSC1 KO HAP1 cells, 3) conditional knockout of TSC1 in mouse brain and 4) TSC1 KO MEFs. The gene discussed is TSC1; the disease is urinary bladder carcinoma.