Mutation or absence of TSC1 led to hypoacetylation of Hsp90 and this was demonstrated in Hsp90 isolated from four cell types lacking TSC1: 1) bladder cancer cells with TSC1 mutation, 2) TSC1 KO HAP1 cells, 3) conditional knockout of TSC1 in mouse brain and 4) TSC1 KO MEFs. This evidence concerns the gene HSP90AA1 and urinary bladder carcinoma.