RPPH1 and lentivirus infection: First, we established three THP-1 cell lines and three HL-60 cell lines with stable knockdown of RPPH1 by shRNA lentivirus infection and screening with the antibiotics.As shown in Figure 2A(Fig. 2), the shRNA construct sh-RPPH1-1 rendered more reduced expression of RPPH1 transcript in THP-1 and HL-60 cells than the other two constructs sh-RPPH1-2 and sh-RPPH1-3.