Regarding to our observations in protective studies, this regimen (L1 + L2 DNA construct: G3) could confer further protection against C3 tumor-challenged mice (survival rate: ~66.67%) depending on stimulation of CD4+ T cell-dominated Th1 responses as well as Granzyme B secretion (indicating CTL activity) as compared to the L1 or L2 DNA constructs, alone (survival rate: ~33.33%). This evidence concerns the gene CD4 and neoplasm.