In the development of obesity, there is greater infiltration of immune cells in this tissue, particularly macrophages, which show increased expression of TLR4.90 Free fatty acids bind to TLR-4, activating JNK and IκB kinase (IKK).91 Because IRS-1 are target of both enzymes, this process affects tyrosine phosphorylation, resulting in reduced GLUT4 translocation.92 This evidence concerns the gene TLR4 and obesity disorder.