A Volcano plot of the data revealed that genes involved in lipid mobilization and adipose thermogenesis (such as Lipe, Nr1d1, Oplah, Nfkbia, Pck1, Cebpb, Vegfa, Angptl4) were increased, and genes correlated with obesity and adiposity such as (Nrip1, Lpl, Zbtb20, Acss2) were decreased in iWAT adipocytes from AdIL10RαKO mice compared to controls (Figure 5D). This evidence concerns the gene OPLAH and obesity due to melanocortin 4 receptor deficiency.