Up‐regulated TYMS by hsa‐miR‐99a and down‐regulated SASH1 by hsa‐miR‐21 were detected in CIN versus N and CIN versus CSCC in our study, indicating its important role in CIN, and up‐regulated TYMS and down‐regulated SASH1 might serve as a biomarker for CIN and the progression of CIN into CSCC. Here, SASH1 is linked to cervical squamous intraepithelial neoplasia.