We obtained reasonable correlations for nuclear and cytoplasmic staining when comparing chromogenic singleplex IHC with fluorescent mIHC results for five known colorectal cancer markers (CDX2, SOX2, SOX9, E-cadherin, and β-catenin), considering that the scoring methodologies are inherently different and that the tissue sections used to compare these methodologies were not neighboring sections in the TMA. Here, SOX9 is linked to colorectal cancer.