By analyzing both serial stains and the multiplex staining of CDX2 and SOX2 we confirmed their inverse relationship in colorectal cancer [16]; however, the inverse correlation was considerably stronger for DIA (Spearman’s rho test, Allred r = −0.16; DIA r = −0.51; n = 357; Fig. 5a). This evidence concerns the gene SOX2 and colorectal cancer.