SOX2 and neoplasm: All five markers are related to tumor differentiation and tumor stemness, and expected to be largely expressed in both normal mucosa (except for SOX2) and in epithelial cancer cells of the colorectum, and only infrequently in stromal cells; CDX2, SOX2, and SOX9 staining were expected to localize to the nucleus and to some degree also to the cytoplasm, E-cadherin was expected to localize to the membrane and the cytoplasm, and β-catenin should frequently be expressed in all three cellular compartments (proteinatlas.org).