CDH1 and colorectal cancer: We obtained reasonable correlations for nuclear and cytoplasmic staining when comparing chromogenic singleplex IHC with fluorescent mIHC results for five known colorectal cancer markers (CDX2, SOX2, SOX9, E-cadherin, and β-catenin), considering that the scoring methodologies are inherently different and that the tissue sections used to compare these methodologies were not neighboring sections in the TMA.