In addition, we demonstrated that the anti-PD-1-scFv and anti-PD-L1-scFv in the corresponding supernatants harvested after adenovirus infections for 2 or 4 days were functional and successfully stimulated high levels of IFN-γ after adding into anti-CD3 stimulated murine splenocytes for 3 days (Fig. 4f). Here, CD274 is linked to adenoviridae infectious disease.