By using flow cytometer, we found a greater proportion of IFN-γ+ and Ki-67+ cells among the tumor filtrating CD8+ T cells after the treatment with synthetic OV expressing mGM-CSF or anti-PD-1-scFv compared to the treatment with the OV expressing EBFP or the non-replicating Ad-GFP virus (Fig. 6d). This evidence concerns the gene CSF2 and neoplasm.