Similarly, SMAD4 nuclear expression was observed in 20.0% (±22.6) and 2.6% (±5.9) (p < 0.001) of tumor cells with cytoplasmic pAMPK positivity and negativity, respectively, and in 19.0% (±23.2) and 5.4% (±10.5) (p < 0.001) of those with positive and negative nuclear pAMPK immunostaining, respectively (Figure 3B). Here, SMAD4 is linked to neoplasm.