The results of ISH and IHC demonstrated that ZEB1‐AS1 and ZEB1 expressions in ESCC tissues (positive ratio: 44.6% and 41.1%) were both higher than those in normal tissues (14.3% and 12.5%) (Figure 2A‐D), which were also confirmed by qPCR (Figure 2E,F). Here, ZEB1 is linked to esophageal squamous cell carcinoma.