Subsequent investigation uncovered that the relative levels of ZEB1‐AS1 in ESCC cells (EC9706, Eca109, TE1, Kyse70 and Kyse450) were markedly higher than those in Het‐1A cell (P < .01), in which EC9706 and TE1 cells exhibited the highest ZEB1‐AS1 level (Figure 4C). Here, ZEB1-AS1 is linked to esophageal squamous cell carcinoma.