It is well documented that antisense lncRNAs control the level of the sense genes directly or indirectly.29 ZEB1‐AS1 is an antisense cognate gene of ZEB1, and we put forward that whether ZEB1‐AS1 can regulate ZEB1 expression, whereas whether ZEB1 overexpression can reverse the biological process mediated by ZEB1‐AS1 down‐regulation in ESCC cells. The gene discussed is PTGDR; the disease is esophageal squamous cell carcinoma.