The results indicated that ZEB1‐AS1 down‐regulation evidently suppressed tumour growth and reduced tumour weight, whereas ZEB1 overexpression partly recovered the inhibitory effect mediated by pLVX‐shRNA‐ZEB1‐AS1 in ESCC EC9706 and TE1 cells xenografted nude mice (Figure 8A‐D). This evidence concerns the gene ZEB1 and esophageal squamous cell carcinoma.