Further investigation showed that si‐ZEB1‐AS1 strikingly restrained cell proliferation and invasion ability, whereas ZEB1 overexpression obviously reversed the inhibitory effect of proliferation and invasion elicited by si‐ZEB1‐AS1 (Figure 7C‐G), suggesting ZEB1‐AS1 plays a vital regulatory role in cell proliferation and invasion by manipulating ZEB1 expression in ESCC cells. The gene discussed is PTGDR; the disease is esophageal squamous cell carcinoma.