To explore the potential role of ZEB1‐AS1 in tumorigenesis in ESCC cells xenografted nude mice, ESCC EC9706 and TE1 cells stably expressing shRNA‐ZEB1‐AS1 or shRNA‐NC were subcutaneously inoculated into the back of nude mice, and tumour volume and weight were measured. This evidence concerns the gene ZEB1 and esophageal squamous cell carcinoma.