Our data demonstrated that ZEB1‐AS1 and its cognate gene ZEB1 were both unregulated in ESCC tissues, and the up‐regulation of ZEB1‐AS1 and ZEB1 were both associated with TNM staging, lymph node metastasis and poor prognosis of patients with ESCC. This evidence concerns the gene ZEB1 and esophageal squamous cell carcinoma.