Furthermore, the combination of thiostrepton and gemcitabine suppressed expression of stemness markers, such as Sox2, β‐catenin and EMT transcription factor Slug in both CL141 and CL152 cells (Figure 4D), indicating that the combination of thiostrepton and gemcitabine suppresses stemness‐ and EMT‐related profiles in both adenocarcinoma and squamous cell carcinoma cells. Here, SNAI2 is linked to adenocarcinoma.