APP and familial Alzheimer disease: We previously developed a 3D human neural cell culture model of AD in which human‐origin neural progenitor cells (NPCs) expressing amyloid precursor protein (APP) or APP/presenilin 1 (PSEN1) with familial AD (FAD) mutations grow to maturity in a 3D culture system (referred as “3D AD” culture).10, 11 Our 3D AD culture exhibits key events in AD pathogenesis, including extracellular aggregation of Aβ and accumulation of hyperphosphorylated tau.