We previously reported a unique strategy for recapitulating AD pathology, namely Aβ‐driven neurofibrillary tangles, in a human NPC‐derived 3D Matrigel culture system.10, 11 Using our 3D system, we found that ReNcell VM human NPCs (ReN cells, Millipore) expressing FAD mutations in the APP gene (ReN‐GA cells) and APP/PSEN1 genes (ReN‐mGAP cells) cause robust extracellular deposition of amyloid plaques and also lead to tauopathy. Here, PSEN1 is linked to Alzheimer disease.