We previously developed a 3D human neural cell culture model of AD in which human‐origin neural progenitor cells (NPCs) expressing amyloid precursor protein (APP) or APP/presenilin 1 (PSEN1) with familial AD (FAD) mutations grow to maturity in a 3D culture system (referred as “3D AD” culture).10, 11 Our 3D AD culture exhibits key events in AD pathogenesis, including extracellular aggregation of Aβ and accumulation of hyperphosphorylated tau. Here, PSEN1 is linked to Alzheimer disease.