Interestingly, ectopic expression of dominant-negative AMPKα (AMPKα-dn) by infection with Ad-AMPKα-dn partially prevented GLPT from inhibiting mTORC1-mediated S6K1 and 4E-BP1 phosphorylation (Fig. 5d), indicating that GLPT indeed inhibits mTORC1 partly through activation of AMPK. This evidence concerns the gene EIF4EBP1 and infection.