Subsequently, we pretreated the cells with 3-methyl adenine (3-MA) for 3 hours before exposure to ADM, and we observed that both of LC3-II/LC3-I ratio and Beclin1 level were significantly suppressed in K562/ADM and K562 cells (Figures 4(a) and 4(b)), reconfirming that ADM induces autophagy in AML cells which can be prevented by the autophagy inhibitor. This evidence concerns the gene BECN1 and acute myeloid leukemia.