The parasitic form tested in our study for all the strains used was the metacyclic, the same as that of the natural infections in vector transmission, and we observed a better expression of TLR2 in cells infected by the CL-Brener strain belonging to TcVI in relation to the INC5 strain belonging to TcI and a better expression of TLR4 in relation to the Ninoa strain in the TcI group. The gene discussed is TLR4; the disease is infection.