Based on the data demonstrating increased expression of the EP4 protein in LMS compared to normal myometrium and leiomyoma and on the account that EP4 has not been previously explored in LMS, we evaluated the total protein expression of EP4 (Figure 4B) and class III β-tubulin (Figure 4A) in uterine sarcoma (SK-UT-1), uterine carcinoma (SK-UT-1B), and immortalized normal myometrium (PHM1-41) cell lines. The gene discussed is PTGER4; the disease is leiomyoma.