IHC was carried out on tumor biopsies to determine the regulation of NF-κB regulated genes and inflammatory biomarkers, such as p65, cleaved caspase-3 (CC-3), matrix metallopeptidase-9 (MMP-9), histone acetyltransferase p300, cyclin-dependent kinase inhibitor p21, and vascular endothelial growth factor (VEGF), on standalone-treated and drug-combination-treated 4T1 tumor biopsies. This evidence concerns the gene NFKB1 and neoplasm.