The combination of autophagy activators and inhibitors together have also been studied; CQ and HCQ potentiate the effect of mTOR inhibitors, such as temsirolimus [228] or everolimus in colorectal cancer cells [229], melanoma [228], and neuroendocrine neoplasms [230], showing inhibition of autophagy as a mechanism to overcome resistance to mTOR inhibitors. This evidence concerns the gene MTOR and colorectal cancer.