Our results demonstrate that this diagnostic approach permits the screening of hemoglobinopathies in patients with heterogeneous clinical phenotype, as in this case of chronic hemolytic anemia characterized by microcytosis, hypochromia, normal HbA2, and HbF that was not diagnosed by the common first level protocols of investigation for congenital hemolytic anemias. Here, HBA2 is linked to hemoglobinopathy.