In addition, given one of the well-documented mechanisms in TMZ response involves elevated methylation of O6MeG DNA methyltransferase (MGMT) (Yan et al., 2016), we further want to evaluate whether the ADAMTS9-AS2 modulated TMZ-resistant behaviors was dependent on the MGMT methylation status in different GBM cells. This evidence concerns the gene ADAMTS9 and glioblastoma.