SLFN11 and prostate carcinoma: To examine potential epigenetic mechanisms for regulation of SLFN11 gene expression by CD47 in prostate cancer cells, we performed chromosome immunoprecipitation in WT and CD47-null PC3 cells using acetylated H3K18, trimethylated H3K4, and trimethylated H3K27 antibodies and analyzed their enrichment in a region upstream from SLFN11 that was identified based on ENCODE data to contain a high abundance of histone H3K27Me3 modification (Supplementary Figure 5).