To explore its exact role in cancer metastasis, CRC cell lines HCT116 and SW480 were infected with the lentivirus containing either shRNA targeting AZGP1 (shAZGP1) or a control scrambled shRNA (shCON), the efficiency of AZGP1 knockdown was verified by Western Blot (Fig. 2A). This evidence concerns the gene AZGP1 and cancer.