To explore the role of AZGP1 in liver metastastic colorectal cancer, firstly, we verified the mRNA expression by qPCR, the result showed that AZGP1 was more abundant in primary tumors with liver metastasis than those without liver metastasis, besides, in metachronous metastasis primary tumors, it was higher than simultaneous metastasis tumor, although there was no statistical difference (Fig. 1A). This evidence concerns the gene AZGP1 and neoplasm.