Representative photographs for immunostaining were shown in Fig. 1A. Only a total of 16.8% (22/131) was high expression in NPE tissues, while a total of 64.1% (164/256) was high expression in NPC tissues(X2 =77.564, P < 0.001), suggesting CALR protein expression significantly increased in NPC tissues. The gene discussed is CALR; the disease is nasopharyngeal carcinoma.