In the current study, we found that knockdown of CALR could significantly suppress the activation of Stat3 signaling pathway in CNE2 and HONE1 cells, suggesting CALR could activate Stat3, CALR-Stat3 signaling pathway has been involved in the initiation and progress of NPC and the inhibitory roles of LPLUNC1 in NPC, which will be further investigated in the future. This evidence concerns the gene BPIFB1 and nasopharyngeal carcinoma.