Silencing Wnt3 expression in CRC cells reduced the transcript levels of β-catenin (encoded by CTNNB1) and downstream genes including c-Myc and CCND1 (Figure 5C and Supplementary Figure 3B) and led to an increased proportion of p-β-catenin (Supplementary Figure 2), revealing the mechanism of inhibited cellular proliferation and migration capacities and slower tumor growth in vivo. The gene discussed is CCND1; the disease is neoplasm.