We observed that Wnt3 deficiency inhibited glycolysis through suppressing the glucose uptake and the expression of crucial genes controlling cellular glycolytic rate in cancer cells, such as GLUT1, HK2, LDHA, and PKM2. We speculated that alteration of these genes in Wnt3-deficient cells is partially due to the silencing of c-Myc. The gene discussed is HK2; the disease is cancer.