CLDN5 and hydrops fetalis: While some BBB studies have reported decreased tight junction protein expression (Kanoski et al., 2010; de Aquino et al., 2018; Mamo et al., 2019) and reduced capillary density in the CA1 region of the hippocampus of HF-fed animals (Tucsek et al., 2014b), we did not observe differences in total levels of ZO-1 or claudin-5 or in vessel density between C/C and C/HF mice.