Different tricistronic vectors expressing GM-CSF, IL-4 and antigens for engineering of induced DCs (iDCs) were validated, e.g., co-expressing the tyrosinase related protein-2 as a melanoma antigen [44, 45], expressing the phosphoprotein 65 (pp65) human cytomegalovirus (HCMV) antigen [46, 47] or expressing a truncated WT1 (tWT1) as a leukemia antigen [48]. Here, CSF2 is linked to melanoma.