To address this more fully, we investigated Jurkat cells transduced with Vγ4 TCRs—either hu12-γ containing the H-J1 motif identified in active celiac disease IELs (Mayassi et al., 2019) or hu20-γ, a Vγ4 chain lacking the H-J1 motif (non-H-J1)—bearing a diverse range of Vδ1 chains (Melandri et al., 2018) (CDR3 ranged from 12 to 24 amino acids in length) for their capacity to upregulate CD69 and downregulate both TCR and CD3 in response to BTNL3.8-expressing cells. This evidence concerns the gene CD69 and celiac disease.