CD20+ B cells and CD56+ NK cells were rare via IHC staining of HCC tumors and surrounding liver tissue; in particular, the CD56lowCD16+ NK cell subset, typically characterized by enhanced cytotoxicity, are decreased in peripheral blood of HCC patients versus healthy controls and within tumor versus non-tumor liver, a finding associated with more Tregs [94, 97]. This evidence concerns the gene NCAM1 and hepatocellular carcinoma.