MTOR and malignant colon neoplasm: To investigate the impact of drugs on blocking mTOR signaling, we measured, by Western blot, the expression of phospho-S6RPSer235/236 (an mTORC1 effector) in colon cancer cell lines treated for 24 h with rapamycin, AZD2085, and AZD2014, at concentrations ranging from 50 to 500 nM.