Previous studies found that EVOO polyphenols enhanced NO by activating eNOS in the endothelium, decreasing plasma asymmetric dimethylarginine (ADMA) (an inhibitor of NO synthesis), in concomitance with improvement in vascular reactivity, reduced markers of oxidative stress and vascular inflammation (oxidized LDL, adhesion molecules, CRP, etc.), that impairs NO synthesis and bioavailability [108,109,110,111]. This evidence concerns the gene NOS3 and inflammation.