As in the single knockdown results, shMTHFD2 cells decreased by 49.3%, while that of shPAICS cells decreased by 38.4% compared to shLacZ control, suggesting silencing of MTHFD2 and PAICS had synergistic effect on migration ability in MNA neuroblastoma (Fig. 6f). The gene discussed is MTHFD2; the disease is neuroblastoma.