To explore the impact of the two metabolic enzymes on MNA neuroblastoma, we first constructed stable knockdown of LacZ (control), MTHFD2, PAICS, and MTHFD2/PAICS (dual-knockdown) by delivering shRNA hairpins into MNA SK-N-DZ cells followed by puromycin selection (Supplementary Fig. 5). Here, PAICS is linked to neuroblastoma.