According to the impact of MTHFD2 and PACIS on neuroblastoma patient survival, we ascertain their stimulatory effects on cell proliferation by cell viability and colony formation assays in response to MTHFD2 and PAICS expression manipulation (Figs. 1a, 5c–h and Supplementary Fig. 7). The gene discussed is MTHFD2; the disease is neuroblastoma.