In addition, the results also demonstrated that the phosphorylation levels of ERK1/2, JNK, and p38 proteins in the CAMK4 group were significantly lower than those in the control group and NC group (Fig. 9d–g), showing that by inhibiting Ki67 and Bcl-2 and up-regulating the levels of actived-caspase-3 and Bax-2 proteins, upregulation of CAMK4 expression levels regulated proliferation and apoptosis of liver cancer cells. This evidence concerns the gene MAPK8 and liver cancer.