To assess the proportion of Bregs in the OPSCC tumor microenvironment, we analyzed the level of IL-10 secreting TIL-Bs after 5 and 24 h of stimulation with CpG ODN 2006 and CD40L in the presence of PMA, ionomycin and brefeldin A using flow cytometry (Cohort 3). The gene discussed is CD40LG; the disease is neoplasm.