In the present study, although the 4T1 mouse mammary carcinoma cells are known to be poorly immunogenic, Nano-DOX was still found, both in vitro and in vivo, to elicit marked emission of three out of four typical DAMPs, i.e. HSP90, CRT and ATP, only leaving HMGB1 unchanged (Fig. 9). The gene discussed is CALR; the disease is breast carcinoma.