LEF1 and cancer: To determine whether MYC-dependent expression of LEF1 can be generalized to cancer cells derived from tissues other than colon, we silenced MYC in the liver cancer line Huh7 and the pancreatic cancer cell line Colo357 and found that expression of LEF1 was reduced in Huh7 cells, but not as dramatically in Colo357 cells (Fig. 2 f, g).