LEF1 and colonic neoplasm: To determine whether MYC directly binds to the promoter of LEF1 in colon cancer cells, we performed ChIP for MYC in DLD1 colon cancer cells and determined that MYC bound strongly to the conserved E-Box 1 motif present in the LEF1 promoter, but not to E-boxes 2 and 3 or to a non-specific control region 2.6 Kb upstream of LEF1 gene, which we labelled as negative control (NC) (Fig. 2j).