Concerning patients whose samples underwent conventional and ADA diagnostic tests, the PF-ADA investigation elicited adequate sensitivity and high specificity (79.3%, 23/29 and 94.9%, 37/39), followed by pleural biopsy histopathology suggestive of TB (69.2%, 9/13 and 76.2%, 16/21), respectively. This evidence concerns the gene ADA and tuberculosis.