PROM1 and colorectal carcinoma: Cells were maintained under Conditionally Reprogrammed Cell (CRC) protocol conditions using CRC medium (DMEM-F12/FY) containing the ROCK inhibitor Y27632, as described in Materials and Methods [65,66]; this resulted in a population that was stably ~80% CD133(+), even in 2D culture (Figure 1c,d).