Western blot (WB) showed that overexpression of circNSUN2 led to the downregulated expression of the epithelial marker E-cadherin, and upregulated expression of the mesenchymal marker, Vimentin, in CRC cells (Supplementary Fig. 5c), suggesting that circNSUN2 promotes EMT in CRC cells through HMGA2 pathway. Here, HMGA2 is linked to colorectal carcinoma.