Pregnancy in ICR mice is associated with impaired glucose tolerance and increased insulin resistance at both days 10–12 and days 16–18 of pregnancy when compared with nonpregnant controls (Supplemental Figure 1, A–C; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.124540DS1), and this was associated with enhanced glucose-induced insulin secretion, as assessed by significantly higher plasma insulin levels in the pregnant mice 30 minutes after a glucose load (Figure 2B). This evidence concerns the gene INS and Insulin resistance.