<h4>Objective</h4>This study is implemented to probe into the function of lncRNA SBF2-AS1 as a competing endogenous RNA (ceRNA) to sponge microRNA-142-3p (miR-142-3p) in modulating TWF1 expression in the gemcitabine resistance of pancreatic cancer.<h4>Results</h4>LncRNA SBF2-AS1 was highly expressed in pancreatic cancer tissues and cells. This evidence concerns the gene SBF2 and pancreatic neoplasm.