Ultimately, we established a key role of YTHDF3 in m6A-modified GAS5 and degradation of GAS5 transcription, uncovering a negative feedback loop between YAP-interacting lncRNA GAS5 and the m6A reader YTHDF3 in Hippo/YAP signaling and tumor progression of CRC. This evidence concerns the gene GAS5 and neoplasm.