The levels of EVI1-mRNA in the GBM cell lines varied and showed no significant difference; (P = 0.052) (Fig. 2a, left panel), however, the protein levels of EVI1 in GBM cell lines were higher compared with those in normal brain tissues (P < 0.05) (Fig. 2a, right panel, The representative data was shown in Supplementary Fig. 4). The gene discussed is MECOM; the disease is glioblastoma.