MECOM and glioblastoma: To investigate the expression levels of EVI1 in various GBM cell lines, we performed quantitative PCR and western blotting using seven GBM cell lines (i.e., A-172, LN-18, LN-229, T98G, U-87MG, U-251MG, and YKG-1) and compared them with those in normal brain tissues (i.e., NB-1, NB-2, NB-3, NB-4, NB-5, NB-6, NB-7, and NB-8).