When pGL4-377 was co-transfected with the EVI1 overexpression vector into five GBM cell lines (A172, LN229, U-87MG, U-251MG, and YKG-1), exogenous EVI1 enhanced the luciferase activities by 2.9-fold in YKG1 and by 11.4-fold in U-251MG (Fig. 3b). The gene discussed is MECOM; the disease is glioblastoma.