AP5M1 and glioblastoma: To further investigate the correlation between MuD and these genes, we used a GBM cell line, U251-MG, a CRISPR-Cas9-generated MuD KO line β18, a plasmid transfection line containing pEGFP-C1 (C1), and a line SE MuD following MuD-GFP-C1 transfection (C1MuD).