Irrespective of the concentration and duration of the culture, both of the genes were downregulated by CAN, with the exception of GR1, expression of which was at the level of the control after 24 h of CAN at both concentration and GR for 10-μM CAN after 24 h and 50-μM CAN after 72 h (Figure 4C). This evidence concerns the gene GSR and Crouzon syndrome-acanthosis nigricans syndrome.