These immune alterations were positively associated with responsiveness to experimentally-induced melanoma, characterized by: (i) increased numbers of TILs of both the CD4 and CD8 phenotypes; (ii) increased reactivity of these cells, which was associated with enhanced production of IL-2, IFN-γ and tumor necrosis factor (TNF)-γ; and (iii) increased expression of MHC class II molecules, as well as the co-stimulatory molecules, B7.1 and B7.2, by DCs and tumor infiltrating macrophages (TIMs) (86). This evidence concerns the gene CD4 and neoplasm.